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Histopathology Facility Services

Tissue processing/paraffin blocks and sections

Preparation of histological sections from tissues provided by investigators.

Tissue sectioning from frozen blocks

Preparation of OCT frozen block and histological cryosections from tissues provided by investigators.

HE staining and special stain (Trichrome stain, etc.)

H&E and other traditional histological techniques for microscopic evaluation of tissues and cells.

<p><strong>HE staining of mouse multiple organs</strong></p>
<p><strong>HE staining of organoid from mouse esophagus squamous carcinoma</strong></p>
<p><strong>Trichrome staining on breast cancer section</strong></p>

Immunohistochemistry (IHC), since and multiplex IHC staining

Detection of cancer-related proteins in tissue sections. Multiple immunohistochemistry (IHC) assays enable detection of multiple targets in one section.

Single IHC Staining

<p><strong>Single IHC staining (proliferative marker: ki-67 nuclear)</strong></p>
<p><strong>Single IHC staining (membrane marker: Her-2)</strong></p>
<p><strong>Single IHC staining (cytoplasmic marker: pS6)</strong></p>

Available Double IHC panels

<p><strong>PD-L1 (Brown DAB) Ki-67 (Red)</strong></p>
<p><strong>E-Cadherin (Purple) Vimentin (Teal)</strong></p>
<p><strong>pSTAT1 (Purple) HSV1 (Teal)</strong></p>
<p><strong>CD68 (Purple) MERTK (Teal)</strong></p>
<p><strong>CD68 (DAB) MERTK (Red)</strong></p>

Available Triple IHC Panels

<p><strong>CD3 (Yellow)/CD8 (Purple)/Foxp3 (Teal) Triple stain</strong></p>
<p><strong>p53 (Purple)/Pax8 (Yellow)/SV40 (Teal)</strong></p>

Interpretative pathology and histopathology reports

Facility pathologists read slides and report on abnormalities found when requested.

Digital photography

Facility pathologists document microscopic findings when requested

Digital whole slide scan

<p><strong>Facility provides whole slide scanning using Leica Aperio scanner</strong></p>

IHC manual score and digital image analysis

Advanced computer-assisted image analyzers that permit morphometric and densitometric analysis of tissue components and IHC reactions.

<p><strong>Nuclear marker quantitative image analysis (p53 quantification)</strong></p>
<p><strong>Microvessel Density Count (CD31 quantification)</strong></p>
<p><strong>Quantitative image analysis (Lung metastasis quantification)</strong></p>

Tissue Microarray analysis (morphology and IHC evaluation)

Facility pathologists provide the morphology and IHC evaluation of Tissue microarray slide.

<p><strong>Quantitative image analysis on proliferative Index labeled by ki-67 staining on TMA section</strong></p>
<p><strong>Quantitative image analysis on Collagen staining on TMA section</strong></p>

Laser Capture Microdissection

Capable of isolating Normal, Tumor, or Stroma components from either frozen and paraffin sections. Insure the pure cell populations for downstream DNA, RNA and proteomics analysis.

<p><strong>Laser Capture Microdissection of breast tumor cells</strong></p>
This facility suppports Fox Chase Cancer Center's Cancer Center Support Grant (CCSG) from the National Cancer Institute.

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