- Flow cytometry analysis
Cell surface staining using fluorescent-conjugated monoclonal antibodies can be analyzed. Internal determinants can be analyzed if cells are first permeabilized prior to staining.
- Flow cytometry sorting
Cells with desired characteristics (including distinctive protein expression, GFP+) can be isolated.
- Ca++ flux measurement
Measurement of Indo-1 loaded cells enables determination of intracellular calcium ion concentration and alteration after cell stimulation.
The Cell Sorting Facility (CSF) comprises three cytometers: 1) a user-operated BD FACScan analyzer that discriminates 3 analytes and is used for cell cycle analysis; 2) a user-operated BD-LSRII analyzer that discriminates 12 physical or fluorescent parameters and is used for all other analytic applications; and 3) a CSF operator-run BD-FACS Aria II cell sorter, which can discriminate 15 analytes and perform four-stream sorting for physical isolation of cell populations of interest.