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Lu Chen, PhD

About

Assistant Professor

Research Program

Lab Overview

The Lu Chen laboratory (luchenlab.org) studies molecular secrets underlying all things RNA biology – how RNAs are chemically modified, how their exact molecular forms are precisely defined, and how they cooperate with proteins to carry out molecular functions at specific cellular locations. We are translating our basic science discoveries into novel therapeutic options to combat cancer immortality, stem cell exhaustion, and degenerative diseases. We use diverse approaches, including protein-RNA reconstitution, Next-gen RNA structural profiling (icSHAPE-seq, nascent RNA-seq), CRISPR-engineering of human primary stem cell and mouse models, and single-molecule RNA imaging.

 
Education and Training

Educational Background

  • 2020-2021 - Instructor, Stanford Cancer Institute, Stanford Cancer Institute, Stanford, California, USA
  • 2013-2019 - Postdoctoral Fellow, Department of Biochemistry, Department of Medicine-Division of Hematology, Stanford University, Stanford, California
  • 2007-2013 - PhD, Biochemistry and Molecular Biology (with honors), University of Kansas Medical Center, Stowers Institute for Medical Research, Kansas City, Missouri
  • 2002-2006 - BS, Biological Sciences, College of Life Sciences, Wuhan University, Wuhan, China

Honors & Awards

  • 2018 - Postdoctoral Fellowship Award, Stanford Cancer Institute, Stanford, CA
  • 2013 - Honors in Ph.D. thesis and defense, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center
  • 2010 - Best Poster Award at the ASBMB (American Society for Biochemistry and Molecular Biology) Special Symposium, entitled “Transcriptional Regulation by Chromatin and RNA Polymerase II”, Tahoe City, California
Research Profile

Research Program

Research Interests

LuChenLab – RNA biology to solve cancer and other aging-related diseases.

  • Epigenetic mechanisms hijacked by a recurrent somatic noncoding driver mutation.
  • Engineer senescence-resistant CAR-T and other adult stem cells.
  • Develop a DNA/RNA-sensing portable device for cancer detection, powered by CRISPR-Cas and nanotechnologies.
  • Investigate role of chromatin in the life cycle of a ribonucleoprotein.
Lab Staff

Soon-keat Ooi, PhD

Postdoctoral Associate

Room: W246
913-850-2590

Victoria "Tori" Gillespie, BS

Lab Manager/Sci Tech I

Room: W246
215-728-4306
Publications

Selected Publications

Chen L*, Chang HY, Artandi SE. Analysis of RNA conformation in endogenously assembled RNPs by icSHAPE. STAR protocols. 2021 Jun 18;2(2):100477. PMID (*Corresponding author)

Chen L*, Bellone RR, Wang Y, Singer-Berk M, Sugasawa K, Ford JM, Artandi SE. A novel DDB2 mutation causes defective recognition of UV-induced DNA damages and prevalent equine squamous cell carcinoma. DNA repair. 2021 Jan 1;97:103022. PMID (*Corresponding author)

Chen L, Roake CM, Galati A, Bavasso F, Micheli E, Saggio I, Schoeftner S, Cacchione S, Gatti M, Artandi SE, Raffa GD. Loss of human TGS1 hypermethylase promotes increased telomerase RNA and telomere elongation. Cell reports. 2020 Feb 4;30(5):1358-72. PMID

Chen L, Roake CM, Freund A, Batista PJ, Tian S, Yin YA, Gajera CR, Lin S, Lee B, Pech MF, Venteicher AS. An activity switch in human telomerase based on RNA conformation and shaped by TCAB1. Cell. 2018 Jun 28;174(1):218-30. PMID

Chen L, Roake CM, Maccallini P, Bavasso F, Dehghannasiri R, Santonicola P, Mendoza-Ferreira N, Scatolini L, Rizzuti L, Esposito A, Gallotta I. TGS1 controls snRNA 3'end processing, prevents neurodegeneration and ameliorates SMN-dependent neurological phenotypes in vivo. bioRxiv. 2020 Jan 1. doi

Chen L, Ooi SK, Conaway JW, Conaway RC. Biochemical assays for analyzing activities of ATP-dependent chromatin remodeling enzymes. JoVE. 2014 Oct 25(92):e51721. PMID

Chen L, Ooi SK, Conaway RC, Conaway JW. Generation and purification of human INO80 chromatin remodeling complexes and subcomplexes. JoVE. 2014(92):e51720. PMID

Chen L, Conaway RC, Conaway JW. Multiple modes of regulation of the human Ino80 SNF2 ATPase by subunits of the INO80 chromatin-remodeling complex. Proceedings of the National Academy of Sciences. 2013 Dec 17;110(51):20497-502. PMID

Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway JW, Conaway RC. Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. Journal of Biological Chemistry. 2011 Apr 1;286(13):11283-9. PMID (Highlighted in “Milestones in transcription and chromatin published in the Journal of Biological Chemistry”, 2019 Feb 1;294(5):1652-1660.)

Additional Publications

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