Solved Protein Structure Holds Key To Much-Needed Therapies For Metabolic Disorder

February 16, 2016

PHILADELPHIA (February 16, 2016) – Phenylketonuria, also known as PKU, is the most common inherited disease affecting amino acid metabolism. Children are tested for PKU at birth, and babies diagnosed with the disease must adhere to a highly restricted diet to prevent permanent intellectual impairment.  Recent advances reveal a strong need for new therapies to combat cognitive dysfunction and psychiatric disorders that arise due to dietary lapses throughout life.

In a study published February 15th in Proceedings of the National Academy of Sciences, Fox Chase Cancer Center – Temple Health researchers made tremendous strides toward that goal by shedding new light on the structure of phenylalanine hydroxylase (PAH)—the enzyme that is defective in PKU patients. Researchers from Drexel University College of Medicine, the University of Pennsylvania’s Perelman School of Medicine and the University of Canterbury in New Zealand participated in the study.

Eileen K. Jaffe, PhDEileen K. Jaffe, PhD“We have solved the first X-ray crystal structure of full-length PAH, which has defied crystal structure determination for decades,” said senior study author Eileen K. Jaffe, professor of Molecular Therapeutics at Fox Chase. “This structure will help us understand the molecular origins of PKU and is an important advance in developing much-needed drugs for patients.”

PKU is an inherited disorder characterized by low levels of functional PAH, which converts an amino acid called phenylalanine to other important compounds in the body. Defects in PAH cause phenylalanine to build up to toxic levels in the body. Because nerve cells in the brain are particularly sensitive to phenylalanine levels, excessive amounts of this substance can cause defects in brain development or abnormal brain function.

If patients do not strictly follow a low-phenylalanine diet for their entire lives, they risk severe consequences, including seizures, delayed development, behavioral problems, psychiatric disorders, and permanent intellectual disability.  Maintaining this strict diet can be a challenge for people living with PKU – a problem made more difficult by limited development of non-dietary therapies based on knowledge of the structure of PAH.

In the new study, Jaffe and her team overcame substantial technical hurdles to solve the first X-ray crystal structure of full-length PAH. This structure consists of an asymmetric arrangement of four identical subunits in a low-activity form that predominates at low levels of phenylalanine.  Additional solution studies show that a distinct four-unit structure, known as a tetramer, characterizes the activated form of PAH. The evidence that alternate forms of PAH are represented by distinct tetramer structures supports Jaffe’s recently proposed model for the activation of PAH by dietary phenylalanine.

“This study provides the first complete view of the enzyme in a tetrameric form, which was not possible with prior partial crystal structures,” Jaffe said. “It also facilitates the interpretation of a wealth of biochemical and structural data that was previously impossible to evaluate.”

Using what they have learned from this work, Jaffe and her team are now working on solving the structure of activated PAH, which functions to prevent blood phenylalanine from rising to neurotoxic levels. “Knowledge of both PAH structures will help us use structure-based drug design techniques to develop drugs that will favor activated PAH as a therapeutic approach,” Jaffe said.

In the end, this research could pave the way for new treatment strategies not only for PKU, but also for cancer and other diseases. “Both inborn errors of metabolism and cancer occur when our proteins do not function as they should,” Jaffe said. “Our focus on how different protein assemblies can be harnessed to control protein function has tremendous potential for drug discovery. However, this approach requires that protein chemists and pharmaceutical companies embrace an expanded view of how small molecule therapeutics can work.”

The Hospital of Fox Chase Cancer Center and its affiliates (collectively “Fox Chase Cancer Center”), a member of the Temple University Health System, is one of the leading cancer research and treatment centers in the United States. Founded in 1904 in Philadelphia as one of the nation’s first cancer hospitals, Fox Chase was also among the first institutions to be designated a National Cancer Institute Comprehensive Cancer Center in 1974. Fox Chase researchers have won the highest awards in their fields, including two Nobel Prizes. Fox Chase physicians are also routinely recognized in national rankings, and the Center’s nursing program has received the Magnet recognition for excellence five consecutive times. Today, Fox Chase conducts a broad array of nationally competitive basic, translational, and clinical research, with special programs in cancer prevention, detection, survivorship and community outreach. It is the policy of Fox Chase Cancer Center that there shall be no exclusion from, or participation in, and no one denied the benefits of, the delivery of quality medical care on the basis of race, ethnicity, religion, sexual orientation, gender, gender identity/expression, disability, age, ancestry, color, national origin, physical ability, level of education, or source of payment.


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