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Margret B. Einarson, PhD

Margret Einarson, Ph.D.
About

Research Associate Professor

Manager, High Throughput Screening Facility

Research Program

Education and Training

Educational Background

  • PhD, Molecular Biology, Weill Cornell Graduate School of Medical Sciences –Sloan Kettering Institute, New York, NY 1995
  • BS, Bates College, Lewiston, ME, 1988
Research Profile

Research Program

Research Facility

Research Interests

  • The HTSF offers expertise and specialized technology platforms providing support for translational projects within laboratory and clinically based research programs
  • Provide education, expertise and instrumentation for researchers to employ high throughput approaches
  • Assist investigators to execute high throughput screens using small molecule and RNAi libraries to understand and target cellular pathways to identify potential cancer therapeutics
  • Provide support and materials for grant and publication submission

Lab Overview

Assay Services
High Throughput Screening Facility
The HTSF provides equipment, expertise, and direct services that enable investigators to address novel areas of translational research that would be difficult or impossible to pursue without the liquid handling and automated imaging, optimized assays, reagents and experience housed within the Facility.

The HTSF offers expertise and specialized technology platforms providing support for translational projects within laboratory and clinically based research programs

Technology platforms:

BioPlex 200: multiplexed biomarker analyzer
Analysis of multiple markers in a variety of experimental samples (including serum, plasma, cell lysates) yielding quantitative measures of biomarkers of interest (picograms/milliliter).

For more information:
http://www.bio-rad.com/en-us/applications-technologies/bio-plex-multiplex-immunoassays?ID=LUSM0ZMNI

ImageXpress micro Automated microscope and Image analysis
Image Acquisition

Automated microscope for unbiased image acquisition. Images from slides, 96 and 384 well plates. Colors include : DAPI, FITC, Red, Far Red and GFP. Objectives include 4X, 10X, 20X, 40X and 60X

Image Analysis

Images acquired on the IXM or other microscopes can be analyzed with over 10 preset modules including: Count nuclei, Cell scoring, Translocation, Transfluor (dot, pit or vesicle counting).  Metrics returned include measures of intensity and cell morphometrics (cell area, pit/vesicle area, size and shape of cells or objects such as spheroids). Publications from the HTSF utilizing this platform include:

  • Identification of Novel Inhibitors of DLK Palmitoylation and Signaling by High Content Screening.Martin DDO, Kanuparthi PS, Holland SM, Sanders SS, Jeong HK, Einarson MB, Jacobson MA, Thomas GM.Sci Rep. 2019 Mar 6;9(1):3632. doi: 10.1038/s41598-019-39968-8. PMID:30842471
  • Genome-wide siRNA screen reveals a new cellular partner of NK cell receptor KIR2DL4: heparan sulfate directly modulates KIR2DL4-mediated responses. Brusilovsky M, Cordoba M, Rosental B, Hershkovitz O, Andrake MD, Pecherskaya A, Einarson MB, Zhou Y, Braiman A, Campbell KS, Porgador A. J Immunol. 2013 Nov 15;191(10):5256-67. doi: 10.4049/jimmunol.1302079. Epub 2013 Oct 14.
  • Guava EasyCyte
    Benchtop 96-well automated FACS.

    Publication from the HTSF utilizing this platform:
    Identification of a functional network of human epigenetic silencing factors. Poleshko A, Einarson MB, Shalginskikh N, Zhang R, Adams PD, Skalka AM, Katz RA.J Biol Chem. 2010 Jan 1;285(1):422-33. doi: 10.1074/jbc.M109.064667. Epub 2009 Oct 30. PMID:19880521
  • Perkin Elmer Envision Multilabel plate reader
    Plate reader equipped for absorbance, fluorescence, luminescence, FP and BRET and FRET.
    Publication from the HTSF utilizing this platform:
    Anti-Müllerian Hormone Signaling Regulates Epithelial Plasticity and Chemoresistance in Lung Cancer. Beck TN, Korobeynikov VA, Kudinov AE, Georgopoulos R, Solanki NR, Andrews-Hoke M, Kistner TM, Pépin D, Donahoe PK, Nicolas E, Einarson MB, Zhou Y, Boumber Y, Proia DA, Serebriiskii IG, Golemis EA. Cell Rep. 2016 Jul 19;16(3):657-71. doi: 10.1016/j.celrep.2016.06.043. Epub 2016 Jul 7. PMID:27396341
Additional Resources

Compound Libraries, siGenome human library for RNAi screening, liquid handling and automation for plate based assay development.

Publications

Selected Publications

Kiseleva AA, Korobeynikov VA, Nikonova AS, Zhang P, Makhov P, Deneka AY, Einarson MB, Serebriiskii IG, Liu H, Peterson JR, Golemis EA. Unexpected activities in regulating ciliation contribute to off-target effects of targeted drugs. Clinical cancer research : an official journal of the American Association for Cancer Research. 2019.
DOI: 10.1158/1078-0432.CCR-18-3535

Martin DDO, Kanuparthi PS, Holland SM, Sanders SS, Jeong HK, Einarson MB, Jacobson MA, Thomas GM. Identification of Novel Inhibitors of DLK Palmitoylation and Signaling by High Content Screening. Scientific reports. 2019; 9(1):3632.
DOI:10.1038/s41598-019-39968-8

Mancuso P, Tricarico R, Bhattacharjee V, Cosentino L, Kadariya Y, Jelinek J, Nicolas E, Einarson M, Beeharry N, Devarajan K, Katz RA, Dorjsuren DG, Sun H, Simeonov A, Giordano A, Testa JR, Davidson G, Davidson I, Larue L, Sobol RW, Yen TJ, Bellacosa A. Thymine DNA glycosylase as a novel target for melanoma. Oncogene. 2019;,
DOI:10.1038/s41388-018-0640-2

Lulla AR, Slifker MJ, Zhou Y, Lev A, Einarson MB, Dicker DT, El-Deiry WS. miR-6883 Family miRNAs Target CDK4/6 to Induce G<sub>1</sub> Phase Cell-Cycle Arrest in Colon Cancer Cells. Cancer research. 2017; 77(24):6902-6913,
DOI:10.1158/0008-5472.CAN-17-1767

Beck TN, Korobeynikov VA, Kudinov AE, Georgopoulos R, Solanki NR, Andrews-Hoke M,Kistner TM, Pépin D, Donahoe PK, Nicolas E, Einarson MB, Zhou Y, Boumber Y, Proia DA, Serebriiskii IG, Golemis EA. Anti-Müllerian Hormone Signaling Regulates Epithelial Plasticity and Chemoresistance in Lung Cancer. Cell reports. 2016; 16(3):657-71,
DOI:10.1016/j.celrep.2016.06.043

Additional Publications

Bibliography, USNLM

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