The Genomics Facility provides cost-effective, high-quality genomic and epigenetic services and expertise for Cancer Center members. The Research Cytogenetics and Chromosome Microarray Analysis Unit assists in karyotyping, molecular cytogenetic analysis and chromosome microarray analysis. The Expression Microarray and NextGen Sequencing Unit provides microarray platforms for gene and microRNA (miRNA) expression analysis and NextGen sequencing (NGS) solutions for cancer research. The Genotyping & Real Time PCR Unit provides platforms and expertise for targeted DNA and RNA analyses (including quality control, SNP analysis, quantitation of gene expression, quantitative analysis of alternative spliced transcripts, validation of the effectiveness of siRNA transcription silencing, CpG methylation analysis, and preparation of samples for Sanger sequencing by Genewiz and HLA genotyping).
Genotyping and Real-Time PCR*
The Genotyping and Real-Time PCR Core provides various platforms and expertise for genome-wide and targeted DNA and RNA analyses, as well as quantitative real-time PCR services.
Laser Capture Microdissection Facility
Protocols have been developed and optimized for acquisition of high-quality RNA and DNA from both frozen and formalin-fixed, paraffin-embedded tissue. LCM services include slide preparation and staining, and RNA and DNA extraction with qualitative analysis by Bioanalyzer or Nanodrop systems.
Next Generation Sequencing*
The unit operates an Illumina HiSeq2500 sequencer which can perform a full line of high throughput sequencing applications, and can generate 120GB in one day with rapid mode. We currently offer Whole transcriptome sequencing, whole exome sequencing, whole genome sequencing, ChIP-seq, Methylome-seq etc.
The Cytogenetics and Chromosome Microarray Analysis Core provides classical cytogenetics and molecular cytogenetics services, such as mFISH, as well as high-resolution DNA copy number analysis for the detection of genomic imbalances, including chromosomal deletions and amplifications.
* CCSG facilities