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Spectroscopy Support Facility

Director:  Heinrich Roder, PhD
Manager: Takuya Mizukami, PhD
Takuya.Mizukami@fccc.edu
215-728-2841
Location & Phone: Reimann R408
215-728-2841
Pricing & Scheduling: Contact Dr. Mizukmami for pricing and scheduling.

 

Function

The primary functions of the Spectroscopy Support Facility (SSF) are to maintain and operate instrumentation for high-field NMR and fluorescence spectroscopy, to implement and develop methods for biomolecular studies, and to provide technical expertise and assistance to users. A 600 MHz NMR instrument is used for applications such as analysis of complex synthetic compounds, NMR structure determination of peptides, proteins or nucleic acids, studies of protein folding and dynamics using NMR relaxation methods or hydrogen-deuterium exchange, and macromolecular interactions. Investigators can submit samples to facility personnel for routine NMR analysis, or they can be trained to operate the instruments under supervision of facility personnel. The more demanding applications typically are collaborative projects. The facility also houses a scanning fluorescence spectrometer, which is available for a wide range of applications, including ligand binding measurements and studies of macromolecular structure, interaction and assembly.

Description

The 600 MHz NMR spectrometer is fully equipped for high-resolution biological applications, such as heteronuclear, multidimensional and gradient-enhanced experiments. It features a TCI CryoProbe and Bruker Avance Neo console (installed in 2019) with four independent frequency channels for 1H, 2D, 13C, and 15N (TRX1200 transmit/receive units).

The facility also maintains workstations for data acquisition and processing, assignment work and structure calculations. A scanning spectrofluorometer (PTI Quantum Master 2000) features a high-intensity Xe arc light source, a second emission channel, and motorized polarizers for fluorescence depolarization measurements.

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