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This Fox Chase professor participates in the Undergraduate Summer Research Fellowship.
Learn more about Research Volunteering.
Assistant Professor
The Marvin and Concetta Greenberg Pancreatic Cancer Institute
Our research revolves around understanding how the Ras family of small GTPases impinges upon tumor invasion and metastasis, specifically in pancreatic ductal cancer. Ras is a nodal point of signaling, with input and activation from a wide range of stimuli including receptor tyrosine kinases, G protein-coupled receptors, integrins and extracellular matrix interactions, and chemokines. Once activated, Ras in turn triggers several signaling cascades that lead to a variety of transformed phenotypes including loss of cell cycle control, decreased cell death, and metastatic spread. Of particular interest to my lab is delineating how Ras activation in epithelial tumor cells drives signaling crosstalk to other compartments of the tumor microenvironment including stromal fibroblasts, macrophages, and vascular components. Paracrine signaling among these disparate cell populations likely contributes to protection of epithelial cells from cell cycle arrest, apoptosis, and senescence, as well as facilitating dedifferentiation and EMT.
At the same time that crosstalk affects the epithelial cells, there is evidence that aberrant activation of Ras leads to changes in extracellular matrix (ECM). Reorganization and dissolution of ECM by proteases and other enzymes accelerates cell migration for locoregional invasion and distal metastasis. In addition to Ras effects on the ECM architecture, we have recently shown that activation of this family of GTPases in pancreatic cancer leads to expression and secretion of CXC chemokines that is in part MAP kinase dependent. Vascular endothelial cells are sensitive to these chemokines via CXCR2 receptors, and respond by showing increased migration, invasion, and organization into vessel precursors. This angiogenesis may provide not only a means to nourish nascent tumors, but also serve as a conduit for malignant spread. As a result, we are interested in testing the hypothesis that small molecule inhibitors of the CXC-CXCR signaling axis may reduce the invasion and metastasis of pancreatic cancer and/or the expansion of already disseminated micrometastases.
Pancreatic cancer is also characterized by having an extensive stromal component. It is hypothesized that this bulk of fibroid tissue may be one of the reasons pancreatic cancer remains particularly insensitive to chemotherapeutic agents. Another arm of our research attempts to investigate whether tumor fibroblasts, like vascular endothelial cells, are responsive to CXC-CXCR crosstalk signaling triggered initially by epithelial K-Ras activation.
It is well established that mutational activation of K-Ras occurs very early in pancreatic ductal dysplasia and remains an important signaling impetus through the development of malignancy. We are investigating whether the signaling crosstalk between Ras and CXC chemokines is similarly triggered early in cancer progression by screening patient samples of initial dysplasia (pancreatic intraepithelial neoplasia, PanIN) to frank adenocarcinoma. By understanding the progression this signaling, we hope to uncover biomarkers to be used for screening and earlier detection.
As a member of the Marvin & Concetta Greenberg Pancreatic Cancer Institute, our lab focuses on the K-Ras oncogene and its signaling in pancreatic ductal adenocarcinoma (PDAC). While K-Ras is commonly hyperactivated (by mutation or extrinsic upstream signaling) in many cancer subtypes, and activating mutations occur in approximately 95% of PDAC cases, it remains a largely intractable target pharmacologically-speaking. Although novel mutation-specific K-Ras inhibitors are currently in clinical trials, their K-Ras G12C target is infrequent (~2%) in PDAC. As such, we are investigating the signaling cascades driven by K-Ras function to illuminate potential therapeutic targets.
Our research revolves around understanding how the Ras family of small GTPases impinges upon tumor invasion and metastasis, specifically in pancreatic ductal cancer. Ras is a nodal point of signaling, with input and activation from a wide range of stimuli including receptor tyrosine kinases, G protein-coupled receptors, integrins and extracellular matrix interactions, and chemokines. Once activated, Ras in turn triggers several signaling cascades that lead to a variety of transformed phenotypes including loss of cell cycle control, decreased cell death, and metastatic spread. Of particular interest to my lab is delineating how Ras activation in epithelial tumor cells drives signaling crosstalk to other compartments of the tumor microenvironment including stromal fibroblasts, macrophages, and vascular components. Paracrine signaling among these disparate cell populations likely contributes to protection of epithelial cells from cell cycle arrest, apoptosis, and senescence, as well as facilitating dedifferentiation and a hallmark of tumor progression and metastasis, epithelial-mesenchymal transition (EMT).
At the same time that crosstalk affects the epithelial cells, there is evidence that aberrant activation of Ras leads to changes in extracellular matrix (ECM). Reorganization and dissolution of ECM by proteases and other enzymes accelerates cell migration for locoregional invasion and distal metastasis. In addition to Ras effects on the ECM architecture, we have recently shown that activation of this family of GTPases in pancreatic cancer leads to expression and secretion of CXC chemokines that is in part MAP kinase-dependent. Vascular endothelial cells are sensitive to these chemokines via CXCR2 receptors, and respond by showing increased migration, invasion, and organization into vessel precursors. This angiogenesis may provide not only a means to nourish nascent tumors, but also serve as a conduit for malignant spread. As a result, we are interested in testing the hypothesis that small molecule inhibitors of the CXC-CXCR signaling axis may reduce the invasion and metastasis of pancreatic cancer and/or the expansion of already disseminated micrometastases.
Pancreatic cancer is also characterized by having an extensive stromal component. It is hypothesized that this bulk of fibroid tissue may be one of the reasons pancreatic cancer remains particularly insensitive to chemotherapeutic agents. Another arm of our research attempts to investigate whether tumor fibroblasts, like vascular endothelial cells, are responsive to CXC-CXCR crosstalk signaling triggered initially by epithelial K-Ras activation. It is well established that mutational activation of K-Ras occurs very early in pancreatic ductal dysplasia and remains an important signaling impetus through the development of malignancy. We are investigating whether the signaling crosstalk between Ras and CXC chemokines is similarly triggered early in cancer progression by screening patient samples of initial dysplasia (pancreatic intraepithelial neoplasia, PanIN) to frank adenocarcinoma. By understanding the progression this signaling, we hope to uncover biomarkers to be used for screening and earlier detection.
Finally, some of our recent work has demonstrated an interaction between K-Ras and the RNA-binding protein, HuR. Current investigations are looking at how K-Ras activation via genetic mutation influences HuR function and regulation of the cancer progression transcriptome, and in turn, how HuR governs K-Ras-dependent signaling in tumor epithelial cells.
There is an opening for a motivated Postdoctoral Fellow in the research laboratory of Paul Campbell, PhD, in the Cancer Signaling & Epigenetics Program at Fox Chase Cancer Center (FCCC) in Philadelphia. The lab focuses on gastrointestinal cancers, predominantly pancreatic cancer, and the microenvironment signaling that drives cancer progression. We are interested in basic and translational science approaches that ultimately lead to improved therapeutics. The lab is part of the highly collaborative Marvin and Concetta Greenberg Pancreatic Cancer Institute and the associated Pancreatic Cancer Translational Working Group.
The ideal candidate will be engaged in identifying novel mechanisms that promote the development of pancreatic cancer from early lesions through to metastatic disease, using fundamental mechanisms discovered in model organisms as a starting point for discovery. Familiarity with methods for manipulating gene expression and signaling in mamalian cultured cells and mouse models, as well as experience in molecular biology are desired. Please provide a brief summary of future career plans in your application.
Requirements: Applicants should have a PhD in biomedical sciences or related field. This position requires considerable experience in cell and molecular biology techniques, with a strong bias towards candidates with significant cancer research experience. Excellent written and oral communication skills in English are required. Recent or soon-to-be graduates are preferred.
Education: Doctorate Degree - PhD in a Biomedical Sciences field (MD or DVM is acceptable in lieu of PhD)
Experience: General experience and graduate level knowledge of key scientific concepts and molecular biology lab techniques.
As one of the four original cancer centers to receive comprehensive designation from the National Cancer Institute, Fox Chase Cancer Center has been at the forefront of cancer research for almost 90 years. We are home to excellent research facilities, top clinicians and scientists, and outstanding patient care. Our singular focus on cancer, which couples discovery science with state of the art clinical care and population health, remains the foundation of our work.
The scientist training programs at Fox Chase Cancer Center provide professional development opportunities in four core areas identified as crucial for successful careers in science, research, and health care including communication, leadership, teaching, and mentorship. Upon joining the program, graduate students and postdocs develop individual development plans to help guide their growth. Training throughout the year is supplemented with free professional development opportunities, including a robust ‘How To’ series, writing courses, networking, mentorship, and teaching opportunities, a trainee-led seminar series, a trainee-led annual Research Conference, and more. Postdocs at Fox Chase Cancer Center are supported by the Temple University Postdoc Association and the Office of Academic Affairs at Fox Chase, and are compensated with competitive pay and benefits.
In addition to the robust training program, scientists at Fox Chase Cancer Center benefit from being part of the rich scientific and biotech environment in the Philadelphia region. Many of our former trainees are now employees (and contacts) at nearby institutions and companies, including The Wistar Institute, Merck, GSK, AACR, and numerous others.
Interested candidates should send a curriculum vitae, a brief description of research experience, and contact information for three references to Dr. Paul Campbell (email: campbell_labpostdoc@temple.edu).
A full time research technician position is available in a cancer biology laboratory in the Cancer Signaling & Epigenetics Program and the Marvin & Concetta Greenberg Pancreatic Cancer Institute at Fox Chase Cancer Center in Philadelphia. The main interests of the Campbell lab are to understand how signaling from the tumor microenvironment drives incidence and progression of gastrointestinal cancers, predominantly pancreatic cancer.
The successful candidate will conduct, under supervision of the principal investigator, experiments in signaling biology, biochemistry, and molecular pharmacology aimed at understanding the molecular mechanisms of action of cancer invasion with a goal of discovering and validating new therapeutic targets and biomarkers.
In addition, the successful candidate will be responsible for ordering and organizing laboratory supplies, maintaining equipment, cataloging reagents, and other organizational tasks.
It is expected that the individual will present his/her research findings internally and for the motivated candidate, at local and national conferences.
Applicant should have a bachelor’s degree in a basic medical science discipline (physiology, biochemistry, molecular biology etc.) or biology and experience working in a research laboratory is required. Experience in mammalian cell culture, basic molecular biology techniques (transformation, gene subcloning, DNA mini- and maxi-preps, PCR), and basic biochemistry techniques (SDS-PAGE, western blots, immunoprecipitation, immunohistochemistry) is strongly preferred.
Excellent communication skills (verbal and written) and interpersonal skills are required. The successful candidate will be a liaison amongst multiple labs and clinical personnel within our Cancer Center, with collaborators at other research institutes, and with supply vendors.
Excellent organizational skills required: ability to multitask will be essential for success in this position.
As one of the four original cancer centers to receive comprehensive designation from the National Cancer Institute, Fox Chase Cancer Center has been at the forefront of cancer research for almost 90 years. We are home to excellent research facilities, top clinicians and scientists, and outstanding patient care. Our singular focus on cancer, which couples discovery science with state of the art clinical care and population health, remains the foundation of our work.
The scientist training programs at Fox Chase Cancer Center provide professional development opportunities in four core areas identified as crucial for successful careers in science, research, and health care including communication, leadership, teaching, and mentorship. Upon joining the program, graduate students and postdocs develop individual development plans to help guide their growth. Training throughout the year is supplemented with free professional development opportunities, including a robust ‘How To’ series, writing courses, networking, mentorship, and teaching opportunities, a trainee-led seminar series, a trainee-led annual Research Conference, and more. Postdocs at Fox Chase Cancer Center are supported by the Temple University Postdoc Association and the Office of Academic Affairs at Fox Chase, and are compensated with competitive pay and benefits.
In addition to the robust training program, scientists at Fox Chase Cancer Center benefit from being part of the rich scientific and biotech environment in the Philadelphia region. Many of our former trainees are now employees (and contacts) at nearby institutions and companies, including The Wistar Institute, Merck, GSK, AACR, and numerous others.
Interested candidates should send a cover letter describing their previous research experience, a current resume, and a list of three references to: Dr. Paul Campbell at campbell_labtech@temple.edu.
This Fox Chase professor participates in the Undergraduate Summer Research Fellowship.
Learn more about Research Volunteering.
